Search results for “ELISA”

About 23 results in articles

Open Access Pub publishes peer-reviewed, free-to-read open-access articles. Showing articles matching ELISA — open any to read the full text, or download the PDF or XML.

Comparison of an HPLC-MS/MS Method with Multiple Commercial ELISA Kits on the Determination of Levels of 8-oxo-7,8-Dihydro-2'-Deoxyguanosine in Human Urine

Dec 2018 DOI 10.14302/issn.2377-2549.jndc-18-2430
John P. Richie JrCorresponding author

Introduction: Analysis of 8-oxodG is usually conducted by either chromatography-based methods or by immunochemical methods commonly used based upon their low cost and high-throughput. However, concern regarding the accuracy of ELISA methods has complicated their use. We directly compare the levels o…

Camel Brucellosis in Ethiopia: Seroprevalence and Associated Risk Factor

Oct 2023 DOI 10.14302/issn.2575-1212.jvhc-23-4532
Sultan Abda NejaCorresponding author

Camels are a significant source of income for nomadic populations in many developing countries, including Ethiopia. Camels are well adapted to dry and semi-dry regions, providing income, food security, and transportation. However, camel production and productivity are constrained by infectious disea…

Research Gaps in Contaminants of Emerging Concern (CECs): Routes to the Standardization of Chemical Test Methods By GC/LC-Mass Spectrometry: A Review

Jun 2023 DOI 10.14302/issn.2377-2549.jndc-23-4615
Thavrin ManickumCorresponding author

A literature review was undertaken with a focus on 1) identifying the research gaps regarding CECs, 2) identifying the most common ones, and 3) identifying the typical analytical methods/technologies employed, for their analysis. A total of 214 papers were noted, with a total of 21 review articles (…

Baroreflex Sensitivity, Cardiac and Kidney Remodeling and Deterioration in Vasoactive Substances Content in Blood in Experimental Model of Renovascular Hypertension. Action of Natural Flavone, Luteolin

May 2023 DOI 10.14302/issn.2574-4488.jna-23-4545
N.A. PapiashviliCorresponding author

This study aimed to analyze pharmacological actions of phenolic compound luteolin on the renal and cardiac hypertrophy, blood pressure (BP), baroreflex sensitivity (BRS), levels of epoxyeicosatrienoic acids (EETs), prostaglandin E-2 (PGE-2) and endothelin-1 (E1) in plasma in the 2 kidney - 1 clip (2…

Evolution of the Solid Human Tumor Cells Properties in Various Experimental Systems in Vitro

Jan 2022 DOI 10.14302/issn.2372-6601.jhor-22-4061
Anna B. DanilovaCorresponding author

Background Human malignant cell models which reflect the structural and physiological complexity of tumor tissue are of great importance for preclinical research in oncology. Spheroids/tumoroids derived from solid tumors are of great interest as cellular models mimicking the first vascular-free grow…

Cytokine Expression in Peripheral Blood Mononuclear Cell Cultures Obtained from Cattle with Different Stages of Natural Mycobacterium bovis Infection

Dec 2021 DOI 10.14302/issn.2575-1212.jvhc-21-4034
Fernando Díaz-Otero,Corresponding author CENID-Salud Animal e Inocuidad. Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias (INIFAP). Carretera México-Toluca, Km. 15.5, C.P. 05110, Ciudad de México, México.

In bovine tuberculosis (bTB), cellular, humoral, or both types of immune responses have been observed. The purpose of this study was to examine the immune status of tuberculous cows based on the differential cytokine gene expression associated with Th1 (IFN-γ, IL-2), or Th2 (IL-4, IL-10) responses.…

The Evolution of the Enzyme Immunoassay/Enzyme-Linked Immunosorbent Assay

Aug 2021 DOI 10.14302/issn.2326-0793.jpgr-21-3917
Leonid TarassishinCorresponding author

50 years ago the Enzyme Immunoassay Enzyme-Linked Immunosorbent Assay, mostly known as ELISA was developed. This is a powerful but simple method that is very widely used in the diagnostic practice, as well as in biomedical research. During this time a number of ELISA modification were developed that…

Evaluation of Inflammatory Serum Cytokines after Treatment with the Consciousness Energy Healing Based Proprietary Test Formulation on Combination of Cecal Slurry, LPS and E. Coli Induced Systemic Inflammatory Response Syndrome (SIRS) in Sprague Dawley Rats

Jul 2021 DOI 10.14302/issn.2766-8681.jcsr-21-3885
Snehasis JanaCorresponding author

Sepsis is a systemic inflammatory response to a confirmed or suspected infection. The transition from sepsis to septic shock causes high rate of mortality. The aim of this experiment was to evaluate the anti-inflammatory potential of the Biofield Energy Treated (Blessed) Proprietary Test Formulation…

Evaluation of Renal and Cardioprotective Potential of the Biofield Energy Treated Proprietary Test Formulation on L-NAME and High Fat Diet-Induced Cardiovascular Disorders in Sprague Dawley Rats

Jun 2021 DOI 10.14302/issn.2574-4488.jna-21-3847
Snehasis JanaCorresponding author

The aim of this study was to evaluate the impact of Biofield Energy Treated/Blessed Proprietary Test Formulation and Biofield Energy Treatment/Blessing per se on kidney biomarkers on L-NAME and high fat diet (HFD)-induced cardiovascular disorders in Sprague Dawley rats. In this experiment, the funct…

Generation of a Single-Domain Antibody against Isolated Escherichia Coli that Causes Camel-Calf Death

Mar 2021 DOI 10.14302/issn.2575-1212.jvhc-21-3767
Faysal Abdishakur HassanCorresponding author

Antibodies and antibody fragments, especially single-domain antibodies known as nanobodies, are important tools in diagnostics, research, and therapeutics. In a conventional antibody, light and heavy chains contribute to the formation of the antigen binding site. In addition to conventional antibodi…

The Feasibility of Enzyme Immunoassay Tests in the Absence of a Conventional Source of Electricity

Oct 2020 DOI 10.14302/issn.2576-6694.jbbs-20-3517
Camille S.U. DOSSOUCorresponding author

Background of the Study African  countries are facing frequent blackout. Thus in sub Saharan region, due to frequent power cut, the laboratory professionals find sometimes difficulty to carry out earlier diverse diagnostic tests. Objective The  aim of this work is to evaluate the feasibility of enzy…

Molecular Study of Hepcidin HAMP (-582A/G) Gene Polymorphisms and Measurement of Serum Hepcidin Level among Sudanese Patients with Anemia of Chronic Kidney Disease

May 2020 DOI 10.14302/issn.2575-7881.jdrr-20-3343
Amged Hussen AbdelrhmanCorresponding author

Background Anemia of chronic disease is anemia found in certain chronic disease states, is typically marked by the disturbance of iron homeostasis or hypoferremia. Chronic renal failure is currently known as Chronic Kidney Disease (CKD) or Chronic Renal Insufficiency (CRI) implies long-standing, pro…

RETRACTED: Assessment of the Risk of Hemochromatosis in Polytransfused Sickle Cell Patients at the Abidjan Transfusion Therapy Unit

Apr 2020 DOI 10.14302/issn.2372-6601.jhor-20-3189
Mamadou Sekongo YassonguiCorresponding author Department of Training and Research, National Blood Transfusion Center; Abidjan; Côte D’Ivoire

This article has been retracted on 29 January 2021. VIEW THE RETRACTION NOTICE (https://doi.org/10.14302/issn.2372-6601.jhor-25-5854) In Côte d'Ivoire, sickle cell disease affects 14% of the population. It is responsible for significant morbidity and mortality. Transfusion is a significant element i…

Interest of Confirmation Tests in the Diagnosis of Viral Hepatitis C to Blood Donors in Abidjan-Côte d'Ivoire

Jan 2020 DOI 10.14302/issn.2372-6601.jhor-20-3186
Yassongui Mamadou SekongoCorresponding author

Introduction The anti-HCV RIBA test verifies the presence of anti-HCV serum antibodies detected by the Elisa test. In Côte d'Ivoire, screening for hepatitis C is done exclusively by enzyme immunoassays. In order to reduce the number of HCV positive blood donor exclusions on ELISA, we conducted this…

Effect of the Biofield Energy Treated Proprietary Test Formulation for Sleep Biomarkers in the Unpredictable Chronic Stress (UCS) Animal Model 

Jan 2020 DOI 10.14302/issn.2474-9273.jbtm-19-3157
Snehasis JanaCorresponding author

Sleep biomarkers in brain such as melatonin, BDNF (Brain-derived neurotrophic factor), PGD2 (Prostaglandin D2), leptin, orexin-A, and acetylcholine were evaluated in the unpredictable chronic stress (UCS) rodent model in the presence of Consciousness Energy Healing Treated (the Trivedi Effect®) nove…

Effect of Oral Honey Consumption on Immune Function Following Ex-Vivo Lipopolysaccharide Stimulation in Healthy Humans

Mar 2019 DOI 10.14302/issn.2379-7835.ijn-19-2639
Olivia McCarthyCorresponding author

Nine healthy individuals with a mean ± SD BMI of 22.0 ± 0.7 kg/m² and age of 20 ± 0.2 years, participated in this single-blind randomised, crossover trial investigating the impact of ingesting two different honeys (1) Tropical Forest Honey (TFH) and (2) Manuka Honey; strength 12+ (MAN) on circulatin…

Effect of Coinfection by Fasciola hepatica and Mycobacterium bovis on Bovine Tuberculosis Immunodiagnosis in an Enzootic Area Hidalgo State, Mexico.

Dec 2018 DOI 10.14302/issn.2575-1212.jvhc-18-2487
Fernando Díaz-OteroCorresponding author Centro Nacional de Investigaciones Disciplinarias en Microbiología Animal, Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias. Carretera México-Toluca, Km. 15.5, C.P. 05110, México D.F.

Parasitic infection by the Fasciola hepatica (F. hepatica) promotes susceptibility towards other infections, such as Mycobacterium bovis. As consequence, could affect diagnostic tests for this disease. Hence, the objective of this study was to assess the impact of F. hepatica coinfection on the most…

Substrate Stiffness Influences the Time Dependence of CTGF Protein Expression in Müller Cells

Jan 2018 DOI 10.14302/issn.2578-8590.ipj-17-1910
William J FosterCorresponding author

Following ocular trauma and retinal detachment, gliotic changes in the retina may develop over the subsequent month, a process known as PVR (proliferative vitreoretinopathy). There have been no successful therapeutic interventions to inhibit PVR. The protein CTGF (Connective Tissue Growth Factor) ha…

Advanced Virological And Clinicopathological Studies On Cattle Suffering From Foot And Mouth Disease Virus

Sep 2017 DOI 10.14302/issn.2577-137X.ji-17-1736
Samia Ahmed KamalCorresponding author

Some strains of Foot and mouth disease virus (FMDV) are endemic in Egypt. The present study was performed on cattle and buffaloes (ages: 3 months up to 1.5 years old, of years 2015 and 2016), which were suffering foot and mouth disease (FMD). Sera and tissues samples were tested by different techniq…

Natural Infection by H1-Like Influenza a Virus in South American Camelids from Argentina: Serological Evidences.

Aug 2017 DOI 10.14302/issn.2575-1212.jvhc-17-1661
Viviana ParreñoCorresponding author

Serum samples from wild and domestic South American Camelids (SAC) from Argentina, collected before (2008), during (2009) and after (2010) the 2009 H1N1 influenza pandemic were tested by hemagglutination-inhibition assay (HIA) to evaluate the seroprevalence of antibodies (Ab) against different subty…

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