Cell culture is the maintenance and propagation of cells in vitro under controlled conditions of medium, temperature, gas composition, and substrate, isolated from the intact organism. Cultures derive from primary tissue explants or from established cell lines, and may be grown as adherent monolayers, in suspension,…
Cell culture is the maintenance and propagation of cells in vitro under controlled conditions of medium, temperature, gas composition, and substrate, isolated from the intact organism. Cultures derive from primary tissue explants or from established cell lines, and may be grown as adherent monolayers, in suspension, or in three-dimensional formats and organoids that better reproduce tissue architecture and microenvironmental cues. By providing a defined and reproducible model system, cell culture allows investigators to dissect cellular function, signaling, proliferation, and differentiation; to test drugs, toxins, and candidate therapeutics; to model disease mechanisms; and to produce biological products such as antibodies and vaccines. Specialized applications include co-culture of immune and target cells, stem-cell expansion and directed differentiation, tissue-engineering constructs seeded onto scaffolds, and three-dimensional wound and tumor models that capture cell-cell and cell-matrix interactions absent from simple monolayers. Careful attention to authentication, sterility, and physiological relevance is essential for valid and translatable results. The journal publishes peer-reviewed research employing in vitro culture across diverse systems, including peripheral blood mononuclear cell cultures, tumor and cancer stem-cell models, neuronal and dopaminergic cell assays, stem-cell and tissue-engineering approaches, and three-dimensional wound-healing models, reflecting the breadth of culture-based cellular and molecular investigation.
12 peer-reviewed articles, ranked by relevance. Each links to its DOI.
The 12 articles above have been cited 346 times in the scholarly literature. Citation data via OpenAlex and Crossref, updated Jun 2026.
A sample of recent works citing this journal's research on Cell Cultures, linking to each citing work.