Kumar Rishikesh, S. Kulshreshtha Pooja, Nanjundiah Vidyanand, S. Kadandale Jayarama, The Chromosomes of Dictyostelium Giganteum, Journal of Chromosomes, Volume 1, Issue 1, 2024, Pages 1-11, ISSN 0000-0000, https://doi.org/10.14302/oap.jc-23-4781. (https://oapgroup.org/jc/article/2143) Abstract: As a first step towards clarifying the basis of the cooperation and conflict seen in chimeric binary mixes of Dictyostelium giganteum, we examined the karyotype of six natural isolates. All six had 5 haploid chromosomes. No meiotic figures were seen. Fluorescence in-situ hybridization was carried out using conserved D. discoideum centromeric DNA sequences as probes. From it, we infer that two chromosomes are sub-metacentric, one is metacentric and two are telocentric. Keywords: Chromosome; Centromere; Dictyostelium giganteum