Mazhar Waqar, Molecular Detection of Mycoplasma Gallisepticum and Mycoplasma Synoviae Infection in Poultry, Journal of Food Science and Hygiene, Volume 1, Issue 2, 2022, Pages 1-5, ISSN 2835-2165, https://doi.org/10.14302/issn.2835-2165.jfsh-22-4263.
(https://oapgroup.org/jfsh/article/1851)
Abstract: In Pakistan, production of poultry has been evolved as a good alternate of beef and mutton. In this study multiplex PCR approaches were adopted to differentiate and detect avian mycoplasma in a single PCR reaction as a step forward in the economization of PCR detection. From the total of 25 samples, 25 (100%) had a positive Mycoplasma isolation result. The biochemical test yielded the highest number of isolations, with MG accounting for 10/25. Commercial DNA-based PCR kits have been developed as a result of recent advancements in diagnostic techniques for Mycoplasma infections. Tetracyclines, fluoroquinolones, tilmicosin, tylosin, spiramycin Infections should be reduced as much as possible.
Keywords: Poultry; PCR detection; mycoplasma; biochemical test