Maria Alves Lívia, Tumang Frare Bruno, Colares Achê David, Lemos de Melo Lamartine, Machado Mendes Mirian, Melo Rodrigues Veridiana, Fernando Moreira Izidoro Luiz, BaMtox, a Myotoxic Enzyme Isolated from the Venom of the Bothrops Alternatus, Journal of Skeletal Muscle, Volume 1, Issue 2, 2022, Pages 1-8, ISSN 2832-4048, https://doi.org/10.14302/issn.2832-4048.jsm-21-3986.
(https://oapgroup.org/jsm/article/1815)
Abstract: Snake envenomations are responsible for a high percentage of deaths, as these toxic proteins induce severe local and systemic effects. In Brazil, the Bothrops genus is responsible for a satisfactory fraction of accidents, including Bothropsalternatus, recognized as urutu, whose venom is capable of inducing severe myotoxicity. In this work, the BaMtox toxin was purified through a combination of three chromatographic steps, ion exchange in DEAE-Sepharose, affinity in Benzamidine Sepharose 6B columns and reversed-phase HPLC chromatography on a C18 column. The BaMtox toxin has a molecular mass of approximately 14kDa and did not show phospholipase activity or hemorrhage. On the other hand, it induced edema and a significant increase in plasma levels of the creatine kinase enzyme. Thus, the protein called BaMtox is able to induce myotoxicity.
Keywords: Snake; snake venom; Bothropsalternatus; BaMtox; myotoxic effect.